The cultivation method of cow tongue mushroom is suitable for clinker bag planting or bottle planting, indoor and outdoor shed mushrooming. The specific methods are as follows.

(1) Cultivation season Choose the conditions of constant temperature 8℃-24℃ and high humidity favorable for mushroom growth. Bull's tongue mushroom can be cultivated generally under the conditions of temperature and humidity to meet its suitable growth. It is usually inoculated in early spring and mushrooms are produced in late spring and early summer season.

(2) Preparation of culture medium Cultivation of raw materials for the Crustacea family copy tree, rice storage and other wood chips, supplemented by drum skin or rice bran, corn flour, sucrose, calcium carbonate. If you use the wood chips of non-Crustacean broadleaf trees, you should also prepare some oak tannin (tannin) to match. The following groups of formulas are commonly used.

Formulation 1: 80% wood chips, 15% wheat bran, 3% cornstarch, 1% sucrose, 1% calcium carbonate, material and water ratio of 1:1.1-1.3, pH 5-5.6 (the same below).

Formulation 2: cottonseed hulls 40%, soybean straw 20%, wood chips 20%, wheat bran 18%, sucrose 1%, gypsum powder 1%.

Formulation 3: corn cobs 30%, cottonseed hulls 25%, miscellaneous wood chips 25%, wheat bran 18%, sucrose 1%, calcium carbonate 1%.

Cultivation bags are made of polypropylene 17cm X 34cm, or wide-mouth glass canning jars (525g). Dosing - bagging (bottles) - sterilization, according to the routine operation.

(3) inoculation culture Sterilized culture bags or bottles, to be cooled to below 28 ℃ for inoculation. Bag planted open the mouth of the bag or not open the side of the head in the bag, playing an inoculation hole, take over the strain. Bottle planted in the bottle material surface to play an inoculation hole, take over the strain. Each bottle of 750 grams of bacteria can be connected to 0-40 bags (bottles). After inoculation placed in the culture room rack culture. Room temperature control 24 ℃ -25 ℃, not more than 28 ℃, not less than 15 ℃ is appropriate. Indoor light, dry, fresh air.

(4) Opening of the base of the fungus bags or vials after 0-40 days of cultivation, the mycelium can be moved to the long mushroom room on the shelf after full-grown, vertical discharge; at the same time, the bag opening will be opened, the film straightened; bottles will be planted to cover the film uncovered, replaced by kraft paper sleeve; and spray water in the air and the mouth of the bag or the sleeve on the paper. This stage requires air relative humidity of 85%, the temperature is maintained at 20 ℃ -22 ℃, keep the air fresh, and give diffused light to induce the emergence of the original base.

(5) Management of mushroom emergence After opening and ventilating to increase oxygen, the white block-like protruding protoplast emerges on the culture medium and gradually grows into tongue-shaped substrate, releasing a large number of light red spores. During the development stage of the fruiting entity, the temperature in the room and shed is about 23℃, the relative humidity of the air should be increased to 90%-95%, ventilation should be strengthened to exclude the carbon dioxide gas, and 500-800 lux of light should be given to promote the normal growth of the fruiting entity. Cow tongue fungus from inoculation to harvest generally 60-70 days, can be taken 2-3 tide, biological conversion rate of about 80%.