However, it must be considered that once the pore size of the gel is smaller than the diameter of the protein -SDS complex, all protein with molecular weight larger than that of the protein still cannot have a good separation effect. If the aperture is too large, all protein can run very fast and cannot be separated.
The key is to see the protein near this molecular weight, such as all protein within 5 KD, and the speed difference should be as large as possible in different concentrations of glue. Assuming lognormal distribution, the variance should be large enough to have a good separation effect. On the other hand, the longer protein travels, the more it can be separated from nearby protein.
Extended data:
When the room temperature is 15 ~ 30℃ and the relative humidity is below 60%, the natural absorption reaction time of coated paper edge is shortened, and one strand is separated in about 15 ~ 30 minutes. If the room temperature is lower than 15℃ (such as 65438+February to March in winter) and the humidity is higher than 60% (summer), the natural absorption reaction time of coated paper edge will be prolonged, generally 30 ~ 50 minutes, in order to achieve the best separation effect.
If the indoor temperature is lower than 15℃ or the relative humidity is higher than 60%, the elbow length of the paper edge after glue brushing will affect the subsequent processing. First, let the paper sample after glue brushing stand for 30 minutes, and blow the paper edge with hot air (be careful not to blow it too close to the glue brushing part to prevent it from cracking), so as to accelerate the rapid absorption reaction and achieve the effect of automatic separation. (You can't blow with hot air immediately after brushing the glue. It takes some time to contact and react to separate the glue from the paper coating. )
When using release adhesive, you must brush the adhesive immediately after trimming the printed matter; After 4 ~ 8 hours, the separation effect will be obviously worse, and the effect will be worse the next day. If the separation effect is not good due to improper coating amount or uneven brushing (unfamiliar operation), it is necessary to cut off the edge of the coated paper again after 1 to 2 mm and brush the separation glue again.
When using release adhesive, it is necessary to ensure that the adhesive is evenly absorbed at the edge of the adhesive tape; Meet the requirements of drying time (if the edge of the paper is wet, checking the separation effect will lead to off-page or cracking). The indoor storage time of insulating glue is generally not less than half a year.
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