For the fragment with the target product size of 80BP, 2.0% agarose gel can be used for electrophoresis. The amount of agarose and TAE is related to the number of samples you run, because the number of samples determines the volume of your gel tank, and the volume of gel tank determines the amount of TAE and tae. For example, if the volume of your gel tank is 20 ml, then if you want to mix 2.0% gel, you need 20 ml.

Pay attention to fully dissolve agarose and boil it when making glue. At the same time, we should also pay attention to the evaporation of TAE during boiling. When the glue was first made, it was boiled in a flask and almost evaporated. Then, don't be hot when adding EB, because above 70 degrees, EB will sublimate and evaporate, and if it is too cold, the concentration of EB will be uneven; Because EB is a carcinogen, we must pay attention to self-protection when operating; Then clean the rubber groove, and the comb teeth must be put straight to avoid irregular running out of the belt.

The gel running time is inversely proportional to the voltage, and the voltage is directly proportional to the distance between the two electrodes of the electrophoresis tank, which is generally less than 5V/cm. Because your fragment is very small, it may be indistinguishable from primer dimer, so when you run electrophoresis again, you should extend the time appropriately, so that the bromophene indicator band runs over two-thirds of the gel, because the dimer will run away after a long time, but the target product will not.