1, the coagulation of polyacrylamide gel is a chemical process, acrylamide monomer is polymerized into polymer through chemical reaction, and the characteristic of chemical reaction is that the higher the temperature, the faster the reaction speed.

2. The coagulation of agarose gel is a physical process, and the long chain of agarose has not changed during the whole process, but the combination of secondary bonds such as hydrogen bonds. The lower the temperature, the stronger the hydrogen bonding.