the sample size of agarose gel electrophoresis generally depends on the size and concentration of agarose gel, as well as the concentration and purity of DNA. Generally speaking, the loading amount of agarose gel electrophoresis should be . 1 to 1. Between μ g.

if the loading amount is too low, DNA bands may be too vague and difficult to read, and accurate results cannot be obtained. If the sample size is too high, the DNA bands will be too dense to be distinguished, which will also affect the accuracy of the results. Therefore, determining the appropriate loading amount is one of the key factors in agarose gel electrophoresis experiment.