1, take materials Fresh small vertebrates, such as fish, frogs, etc., the length of 10 to 20 centimeters, to the living body is good.

2. Fixing. Material washed. If it is a fish, it should be scaled first and then washed. Then, put it into 95% ethanol to fix it for 1 to 2 days. If the material has been preserved with 5 to 10% formaldehyde solution, it should be rinsed with water and then immersed in 95% ethanol to make it fully fixed.

3. Skinning Strip the outer skin of the critter with a dissecting instrument.

4, degreasing. The skinned specimen is tied to a glass slide with a string, organized in a good posture, and immersed in 3% potassium dichromate solution for a few days to remove the dirt and fat particles lumps on the surface of the material. When the solution became cloudy, it was replaced with fresh solution until the solution was not cloudy. Remove the specimen, wash it with water, and then immerse it in 95% ethanol to degrease it for about a week.

5, transparent specimen transferred to 20% potassium hydroxide solution. A week or so, see the muscle is translucent, inside the bone is vaguely visible, that is, the termination of transparency.

6, staining The transparent specimen is immersed in 0.01% alizarin red dye solution for 2 to 3 days, until the bones are stained red.