The temperature range of mycelium development is 9-30℃, and the optimum temperature is 22-24℃. The primordium of fruiting body differentiated at 4- 18℃, and the suitable growth temperature was 10- 14℃. The water content of the culture medium should be about 65%, and water should be replenished before fruiting to make the water content reach 70%-75%. The relative humidity of the mushroom house was 98%- 100% during the mushroom bud differentiation period. The relative humidity of mushroom house during mushroom growth period is 90%-95%; The mycelium growth stage does not need light, but it needs to be stimulated by weak light in the mushroom differentiation stage. The fruiting body grows phototropically. If it is cultivated in the basement or cave, the fluorescent lamp should be on day and night 10- 15 hours. Hypsizygus marmoreus needs fresh air at all stages of growth, especially when a large number of primordia appear, and it should be ventilated 4-8 times per hour; The optimum pH value of mycelium growth stage is 6.5-7.5. Hypsizygus marmoreus is an edible fungus that appears in autumn and winter in the north temperate zone.