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What is the difference between bsa and fbs in cell culture
Bovine serum albumin (BSA), also known as the fifth component, is an albumin in bovine serum added to the enzyme digestion reaction buffer, by increasing the concentration of proteins in the solution, the enzyme protective effect. It prevents the decomposition and non-specific adsorption of the enzyme, can reduce the denaturation of some enzymes, and can reduce the denaturation caused by some unfavorable environmental factors such as heating, surface tension and chemical factors.

Bovine serum albumin (BSA) uses:

1. Adding BSA to the enzyme digestion buffer protects the enzyme by increasing the protein concentration in the solution. Prevent enzyme decomposition and non-specific adsorption, can reduce the denaturation of some enzymes, reduce unfavorable environmental factors such as heating, surface tension and chemical factors caused by denaturation.

2, ELISA is often used, such as containment solution, sample dilution, enzyme conjugate dilution can be used BSA.

3, biochemical research, genetic engineering and pharmaceutical research.

Fetal bovine serum (fbs) is a kind of trait, appearance Light yellow clarified, no hemolysis, no foreign matter slightly viscous liquid.

Fetal Bovine Serum (FBS) functions:

1. Provides hormones that are essential for the maintenance of exponential cell growth, nutrients that are not present in the basal medium or in very small quantities, as well as major low molecular nutrients.

2. Provides binding proteins that recognize vitamins, lipids, metals, and other hormones that bind or modulate the viability of the substances to which they bind.

3, In some cases binding proteins can bind to toxic metals and pyrogenic substances and act as detoxification.

4. It is a source of factors needed for cell attachment and spreading on plastic culture medium.

5, play the role of pH buffer.

6, provide protease inhibitors to inactivate the remaining trypsin during cell passaging to protect the cells from injury.