What is the extraction source of protease K solution? Where are they all used?

English name: protease

SolutionNo.: 39450-0 1-6 Grade: PCR

Grade molecular weight: ~ 18 kda activity:? 600 units/ml, 35℃

Concentration: 20 mg/ml deoxyribonuclease, ribonuclease: none.

Detected features: liquid, a nonspecific serine protease related to subtilisin,

From Candida albicans (.

Wheat genus

Photo album.

), which has high specific activity. Chelating agents such as EDTA or detergents such as SDS can not inactivate protease K, but can enhance and stabilize the activity of the enzyme, which is active in a wide PH range (PH4- 12.5). When nucleic acids are isolated from tissues or cells, nucleases (RNA and DNA) and other enzymes in the reaction are inactivated. Uses: Biochemical research. Broad-spectrum protease, 95C

Heating 10

Minutes, it is completely inactivated. substituted

Diethyl pyrophosphate amide

deal with

ribonucleic acid

Centrifugal tube and gun head are used for extraction to realize inactivation.

ribonuclease

The purpose of, also used to deal with

Water without ribonuclease. Protein molecules in tissues or cells, especially nucleic acids, are fully degraded to obtain auxiliary reagents for releasing and extracting high-quality nucleic acids. It is used for the separation of plasmid or genomic DNA and RNA, and protein polypeptide imprinting experiment. Storage: 2 ~ 8℃

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