After enzyme digestion, the electrophoresis band is a long and bright band. If the spindle is thick in the middle and narrow at both ends, the brightness is enough, indicating that your sample preparation and enzyme digestion are normal. According to marker, see if the distance between the bands you want to distinguish is greater than 1cm. If it is greater than 1cm, the next hybridization can be carried out. If not, run for a while, otherwise the two bands will not be clear after hybridization.